|Intracellular calcium and loss of calcium
Increased ICF Ca+2 results from failure of energy dependent Ca+2-Mg+2 ATPase pump and is also related to increased membrane permeability.
Sustained rise in intracellular Ca2+
But at which stage did the cell actually die? Which is the critical biochemical event (the "lethal hit") responsible for the point of no return? The phenomena consistently proposed to represent irreversibility is:
Mitochondrial membrane disruption leading to irreversible
dysfunction and marked ATP depletion.